Isolasi bakteriofag dari air di lingkungan rumah burung walet menggunakan metode plaque assay

Supriadiansyah, Firman (2024) Isolasi bakteriofag dari air di lingkungan rumah burung walet menggunakan metode plaque assay. Bachelor (S1) thesis, Wijaya Kusuma Surabaya University.

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Abstract

Latar belakang. Bakteriofag adalah virus yang menargetkan bakteri. Bakteriofag memberikan solusi alternatif terhadap masalah infeksi bakteri berbahaya. Bakteriofag dianggap lebih efektif dibandingkan antibiotik. Tujuan penelitian untuk mengisolasi bakteriofag dari air dilingkungan rumah burung walet menggunakan metode plaque assay dan mengetahui agen patogen di dalam air rumah burung walet dimana air tersebut sebagai sumber air minum burung walet. Metode. Jenis penelitian ini merupakan penelitian metode deskrptif labolatorik yakni penelitian bertujuan untuk mengetahui informasi secaraalamiah tentang bakteriofag yang di isolasi dari air lingkungan rumah burung walet. Pengamatan dilakukan dengan Mikroskopis dengan cara mengamti plaque assay pada media TSA yang di isolasi bakteriofag. penelitian ini dianalisis secara deskriptif menggunakan grafik dan tabulasi di inkubasi dengan suhu 30oC. Hasil. Berdasarkan hasil metode spot test, Ditemukan adanya bakteriofag Lysinibacillus sp pada air dilingkungan rumah burung walet saat di inkubasi selama 48 jam dengan suhu 30oC belum muncul plak saat pengamatan akan tetapi disaat inkubasi hari ke 7 terdapat plak pada media diduga Lysinibacillus sp, akan tetapi saat dilakukan uji plaque assay dengan diinkubasi selamat 48 jam dengan suhu 30oC menunjukkan tidak terdapat plaque pada media yang telah diisolasi bakteriofag. Kesimpulan. hasil metode spot test, terdapat bakteriofag Lysinibacillus sp pada air dilingkungan rumah burung walet, akan tetapi penelitian gagal karena tidak dapat terisolasi oleh bakteriofag dengan menggunakan metode plaque assay. Kata kunci : Bakteriofag, air rumah burung walet, Lysinibacillus sp, plaque assay Background. Bacteriophages are viruses that target bacteria. Bacteriophages provide an alternative solution to the problem of dangerous bacterial infections. Bacteriophages are considered more effective than antibiotics. The aim of the research is to isolate bacteriophages from the water in the swallow house environment using the plaque assay method and determine the pathogenic agents in the swallow house water where the water is a source of swallow drinking water. Method. This type of research is a descriptive laboratory research method, namely research aimed at finding out natural information about bacteriophages isolated from the water in the swallow house environment. Observations were carried out microscopically by observing the plaque assay on TSA media in which bacteriophages were isolated. This research was analyzed descriptively using graphs and tabulations in incubation at a temperature of 30oC. Results. Based on the results of the spot test method, it was found that the bacteriophage Lysinibacillus sp was found in the water around the swiftlet's house when it was incubated for 48 hours at a temperature of 30oC, no plaque appeared during observation, but on the 7th day of incubation there was plaque on the media which was suspected to be Lysinibacillus sp, but when it was carried out Plaque assay test with incubation for 48 hours at 30oC showed that there was no plaque in the media from which bacteriophages had been isolated. Conclusion. The results of the spot test method showed that there were Lysinibacillus sp bacteriophages in the water around the swallow house, but the research failed because the bacteriophage could not be isolated using the plaque assay method. Key words: Bacteriophages, swallow house water, Lysinibacillus sp, plaque assay

Item Type:	Thesis (Bachelor (S1))
Uncontrolled Keywords:	Kata kunci : Bakteriofag, air rumah burung walet, Lysinibacillus sp, plaque assay
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General)
Divisions:	Faculty of Vetenary Medicine > Animal Health and Veteriner Public Health Study Program
Depositing User:	2024 Firman Supriadiansyah
Date Deposited:	13 Sep 2024 01:55
Last Modified:	13 Sep 2024 01:55
URI:	http://erepository.uwks.ac.id/id/eprint/18910

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