microRNA-379 as a Candidate Biomarker for Early Diagnosis of Childhood Active and Latent Tuberculosis

Sanjaya, Ayling (2022) microRNA-379 as a Candidate Biomarker for Early Diagnosis of Childhood Active and Latent Tuberculosis. Wolters Kluwer Medknow Publications-India. (Unpublished)

Text 8. microRNA-379 as a Candidate Biomarker for Early Diagnosis of Childhood Active and Latent Tuberculosis.pdf Download (985kB)

Abstract

Background: Childhood active and latent tuberculosis (TB) often go undiagnosed due to limited symptoms and biomarkers. Recently, miRNA has been considered as a candidate biomarker for early diagnosis which can also distinguish between childhood active and latent TB. Aim: This study aimed to identify miRNAs that play a regulatory role in active and latent TB in children, analyzing differences in expression levels and diagnostic values of miRNA-379. Methods: A cross-sectional observational analytic study on children aged 0-15 years having contact history with TB patients was used in this study. Anamnesis, physical examination, tuberculin test, culture, chest X-ray and TB scoring were performed. The samples were divided into three groups: healthy control children, active TB children and latent TB children. miRNA profiling was performed using microarray and miRNA-379 validation was performed using RT-qPCR. The data was analysed to determine miRNAs characterization, expression level and diagnostic value of miRNA-379. Results: Children in active TB-control group expressed 251 miRNA genes comprising 221 down-regulated and 30 upregulated miRNAs.The lowest fold change was observed with miRNA-379 whereas the highest fold change was noticed with miRNA-3613. The latent TB-control group expressed 292 miRNA genes, of which263 miRNAs downregulated and 29 miRNAs up-regulated. Moreover, the lowest and the highest fold change was observed for miRNA-381 and miRNA-3200 respectively.In case of active-latent TB group, 21 miRNA genes were expressed with 2 down-regulatedand 19 up-regulated miRNAs. Amongst these, the lowest fold change was observed for miRNA-379 whereas the highest fold change was observed for miRNA-1299. Expression level of miRNA-379 decreased in controls, decreased even more in latent TB and decreased the most in active TB. The difference in the level of expression of these miRNAs was found statistically significant (p<0.001). Conclusion: miRNA has the potential of being a candidate biomarker for childhood TB. Differences in expression level of miRNA-379 can be used as candidate biomarker to distinguish between latent and active TB in children. Keywords: Childhood tuberculosis, biomarker, expression level, miRNA-379

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine > Medicine Study Program
Depositing User:	Sulimin BP3
Date Deposited:	13 Jan 2023 01:35
Last Modified:	13 Jan 2023 01:35
URI:	http://erepository.uwks.ac.id/id/eprint/13606

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