Potensi ekstrak bajakah tampala (Spatholobus littoralis Hassk) terhadap gambaran histopatologi hepar pada mencit (Mus musculus) yang diinfeksi Escherichia coli

Rangganata, Tomi Rahmat (2022) Potensi ekstrak bajakah tampala (Spatholobus littoralis Hassk) terhadap gambaran histopatologi hepar pada mencit (Mus musculus) yang diinfeksi Escherichia coli. Bachelor (S1) thesis, Wijaya Kusuma Surabaya University.

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Abstract

ABSTRAK Tujuan penelitian ini untuk mengetahui potensi ekstrak bajakah tampala (Spatholobus littoralis Hassk) terhadap gambaran histopatologi kongesti, hemoragi dan sel kupffer pada hepar mencit (Mus Musculus) setelah diinfeksi Escherichia coli. Sebanyak 20 ekor mencit jantan yang digunakan sebagai sampel yang dibagi menjadi 5 kelompok perlakuan dan 4 ulangan. Kelompok tersebut antara lain kelompok kontrol negatif (P0), kelompok kontrol positif (P1), dan kelompok perlakuan yang diberi ekstrak 10% (P2), ekstrak 30% (P3), serta ekstrak 50% (P4) selama 21 hari dengan pemberian sebanyak 0,3mL/hari. Hari ke-29 dilakukan infeksi Escherichia coli 106 cfu/mL sebanyak 1 mL secara intraperitoneal. Sampel organ diambil pada hari ke-32. Potensi ekstrak bajakah dilihat dari hasil gambaran histopatologi melalui perubahan lesi dengan metode skoring. Data yang didapatkan dianalisis dengan uji Kruskal Wallis dan dilanjutkan menggunakan uji Mann-Whitney. Hasil penelitian memperlihatkan perubahan lesi yang paling baik terdapat pada kelompok perlakuan ekstrak 30% (P3) dan ekstrak 50% (P4). Hal ini terjadi karena ekstrak bajakah tampala mengandung senyawa alkaloid, flavonoid, fenolik, saponin dan tanin. Jumlah sel kupffer pada kelompok perlakuan ekstrak 10% (P2), ekstrak 30% (P3), dan ekstrak 50% (P4) meningkat namun tidak berbeda nyata atau tidak signifikan dengan kelompok kontrol positif (P1). Kata kunci: Bajakah Tampala (Spatholobus littoralis Hassk), Hepar, Mencit (Mus musculus), Escherichia coli. ABSTRACT The purpose of this study was to determine the potency of bajakah tampala extract (Spatholobus littoralis Hassk) on histopathological features of congestion, hemorrhage and kupffer cells in the liver of mice (Mus Musculus) after Escherichia coli infection. The sample used was 20 male mice and divided into 5 treatment groups and 4 replications. The groups included negative control (P0), positive control (P1), and a treatment group that was given 10% extract (P2), 30% extract (P3), and 50% extract (P4) for 21 days by giving as much as 0.3 mL/day. On the 29th day of infection with Escherichia coli 106 cfu/mL as much as 1 mL intraperitoneally. Organ samples were taken on day 32. The potency of the bajakah extract was seen from the histopathological results through the lesion changes using the scoring method. The data obtained were analyzed using the Kruskal Wallis test and continued using the Mann-Whitney test. The results showed that the best lesion changes were found in the treatment groups with 30% extract (P3) and 50% extract (P4). This happened because the extract of bajakah tampala contains compounds of alkaloids, flavonoids, phenolics, saponins and tannins. The number of Kupffer cells in the 10% extract treatment group (P2), 30% extract (P3), and 50% extract (P4) increased but was not significantly different or not significantly different from the positive control group (P1). Keywords: Bajakah Tampala (Spatholobus littoralis Hassk), liver, mice (Mus musculus), Escherichia coli.

Item Type:	Thesis (Bachelor (S1))
Uncontrolled Keywords:	Kata kunci: Bajakah Tampala (Spatholobus littoralis Hassk), Hepar, Mencit (Mus musculus), Escherichia coli. Keywords: Bajakah Tampala (Spatholobus littoralis Hassk), liver, mice (Mus musculus), Escherichia coli.
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Vetenary Medicine > Vetenary Education Study Program
Depositing User:	54261 Tomi Rahmat Rangganata
Date Deposited:	28 Sep 2022 00:22
Last Modified:	28 Sep 2022 00:22
URI:	http://erepository.uwks.ac.id/id/eprint/12590

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