Identifikasi cemaran bakteri salmonella sp. pada telur bebek di peternakan kota Surabaya

Muhroni Azis, Khoirul (2022) Identifikasi cemaran bakteri salmonella sp. pada telur bebek di peternakan kota Surabaya. Bachelor (S1) thesis, Wijaya Kusuma Surabaya University.

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Abstract

Penelitian ini bertujuan untuk mendeteksi bakteri Salmonella sp. pada putih telur dan swab kloaka. Total sampel yang diambil sejumlah 48 terdiri dari 24 sampel putih telur dan 24 sampel swab kloaka. pada bebek yang sudah diambil dari peternakan di Surabaya. Sampel dilakukan enrichment, kemudian diisolasi pada media Salmonella Shigella Agar (SSA) dan diinkubasi dengan suhu 37oC selama 24 jam. Hasil positif pada media SSA dan pewarnaan gram kemudian dilakukan uji biokimia pada media Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), Simon’s Citrat Agar (SCA), Urease dan diinkubasi dengan suhu 37oC selama 24 jam. Penelitian ini juga mengunakan kuisioner sebagai instrument penelitian. Hasil penelitian menunjukan terdapat 3 sampel positif Salmonella sp. dari swab kloaka. Hasil analisa faktor resiko, faktor penting yang memepengaruhi adanya bakteri Salmonella sp. yaitu sanitasi kandang. Kata Kunci: Salmonella sp., Telur Bebek, Putih telur, swab Kloaka, Peternakan This study aims to detect Salmonella sp. on egg whites and cloacal swabs. A total of 48 samples were taken consisting of 24 egg white samples and 24 cloacal swab samples. on ducks that have been taken from a farm in Surabaya. The sample was enriched, then isolated on Salmonella Shigella Agar (SSA) media and incubated at 37oC for 24 hours. Positive results on SSA media and gram staining were then carried out with biochemical tests on Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), Simon's Citrate Agar (SCA), Urease and incubated at 37oC for 24 hours. This study also uses a questionnaire as a research instrument. The results showed that there were 3 positive samples of Salmonella sp. of the cloacal swab. The results of the analysis of risk factors, important factors that influence the presence of Salmonella sp. namely cage sanitation. Keywords: Salmonella sp., Duck Egg, Egg white, Cloacal swab, Livestock

Item Type:	Thesis (Bachelor (S1))
Uncontrolled Keywords:	Salmonella sp., Duck Egg, Egg white, Cloacal swab, Livestock
Subjects:	R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
Divisions:	Faculty of Vetenary Medicine
Depositing User:	Khoirul Muhroni Azis
Date Deposited:	07 Sep 2022 00:35
Last Modified:	07 Sep 2022 00:35
URI:	http://erepository.uwks.ac.id/id/eprint/12194

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