The Use of Natural Phenomenon in Obtaining Pregnant Rats and Mice as Experimental Animals with The Same Gestational Ages

Kurniawan Gondo, Harry (2019) The Use of Natural Phenomenon in Obtaining Pregnant Rats and Mice as Experimental Animals with The Same Gestational Ages. UB – Malang. (Unpublished)

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Abstract

Hormonal oestrus synchronization is often used to obtain the same gestational ages animal models, but it may not physiologic nor practical. This research was conducted to find out a simple method in obtaining physiologic pregnant experi-mental animals. We used 120 female rats and mice each, divided into 16 groups with 15 members each. They were classified according to the state of parities, pair or group mating, and synchronization of oestrous. The estrous synchronization is done by utilizing the natural phenomenon (Lee-Boot, Pheromone, and Whitten effect). All nulliparous and multiparous models then were simultaneously mated for one night in pairs (1:1) or in groups (1:5) subsequently. Pregnancy rates were noted based on the number of models delivering pups on day 19-23 post-mating. The highest pregnancy rate was noted in group of multiparous, synchronized and monogamous rats (86.7%), followed by nulliparous, synchronized and monoga-mous rats (73.3%) and multiparous, synchronized and monogamous mice (60%) (p < 0.05). Synchronization of the estrous and monogamous mating of rats or mice had significant roles in pregnancy rates (p < 0.05). To get a high number of preg-nant rats or mice models, we suggest using the multiparous, estrous synchronized and monogamous simultaneous mating within one night. Keywords: Rats, mice, pregnant animal models, oestrus synchronization, LeeBoot Effect

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine > Medicine Study Program
Depositing User:	Sulimin BP3
Date Deposited:	10 Dec 2019 06:06
Last Modified:	10 Dec 2019 06:06
URI:	http://erepository.uwks.ac.id/id/eprint/5340

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