Effect of isothiocyanate therapy on trophoblast cell culture-hyperglycemia atmosphere against Tumor Necrosis Factor- α (TNF-α) and Transforming Growth Factor- β (TGF-β) levels

Gondo, Harry Kurniawan (2023) Effect of isothiocyanate therapy on trophoblast cell culture-hyperglycemia atmosphere against Tumor Necrosis Factor- α (TNF-α) and Transforming Growth Factor- β (TGF-β) levels. Bali Medical Journal.

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Abstract

Background: Diabetes mellitus is becoming a public health problem and can be found in every population, including a pregnant women. Hyperglycemia conditions in pregnancy can lead to gestational diabetes mellitus involving proinflammatory cytokines such as Tumor Necrosis Factor-Alpha (TNF-α) and Transforming Growth Factor- β (TGF-β). Isothiocyanate is a bioactive compound found in Moringa leaf extract with anti-glycemic activity and anti-inflammatory effects. This study aims to evaluate the impact of isothiocyanate therapy on trophoblast cell culture-hyperglycemia atmosphere toward the levels of tumor necrosis factor- α (TNF-α) and transforming growth factor- β (TGF-β). Methods: An experimental study with a post-test-only control group design was conducted using trophoblast cell culture from placental tissue. To create a gestational diabetes event, glucose was administered into the trophoblast cell culture. The sample was divided into six groups: negative control without treatment, positive control by glucose administration, and treatment groups P1, P2, P3 and P4 with the treatment of isothiocyanate with doses of 0.1; 0.2; 0.4 and 0.8 mg/ml, respectively. The TNF-α and TGF-β levels in each group are quantified using immunoassay by ELISA method. Results: The mean TNF-α in the negative control, positive control, P1, P2, P3, and P4 groups were 20.59, 43.54, 32.76, 30.15, 27.32, and 24.05, respectively. While the mean of TGF-β levels in the negative control, positive control, P1, P2, P3, and P4 groups were 15.76, 10.44, 11.05, 11.70, 12.46, and 14.32, respectively. The lowest TNF-α and the highest TGF-β levels were found in the treatment group (P4) that received isothiocyanate with a dose of 0.8 mg/ml. Conclusion: The administration of isothiocyanates from Moringa leaf extract significantly lowered the level of TNF-α and increased the level of TGF-β in hyperglycemic trophoblast cell culture compared with the positive control group.

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine > Medical Doctor Profession Education Study Program
Depositing User:	Sulimin BP3
Date Deposited:	10 Jul 2024 10:10
Last Modified:	10 Jul 2024 10:10
URI:	http://erepository.uwks.ac.id/id/eprint/18375

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