AIR GANDARUSA (Justicia gendarussa Burm. f.) DAN GAMBARAN GEN HYALURONIDASE LEWAT ANALISIS PCR

UTAMI, SRI LESTARI (2013) AIR GANDARUSA (Justicia gendarussa Burm. f.) DAN GAMBARAN GEN HYALURONIDASE LEWAT ANALISIS PCR. Perhimpunan Dokter Spesialis Patologi Klinik Indonesia. (Unpublished)

Text 4. Cek Plagiasi_AIR GANDARUSA (Justicia gendarussa Burm. f.) DAN GAMBARAN GEN HYALURONIDASE LEWAT ANALISIS PCR.pdf Download (1MB)

Abstract

ABSTRACT Gandarusa (J. gendarussa Burm. f.) is an etnomedicine which is used as a male contraceptive alternative, known to inhibit the enzyme function of spermatozoa hyaluronidase during fertilization. One of the ideal contraceptive conditions is the safetyness (among others non mutagenic), requiring a long research during the verification process. The early research is the gene expression of hyaluronidase mice (M.musculus L.) testis given water fraction of the gandarusa with PCR analysis. The total RNA was isolated from the normal mice testis (as the negative control or no treatment group) and the mice testis from the treatment groups. The treatment groups consisted of group I and II treated with water fraction of the gandarusa 15 mg/20 gr BW and 7.5 mg/20 gr BW, subsequently, the positive control group was also given hesperidins 1 mg/20 gr BW once a day per oral during the 1.5 times of spermatogenesis cycle (for 55 days). The results of the study showed that (1) the cDNA fragment confirmed as the gene of hyaluronidase mice testis (with 710 bp length of nucleotide) passed through RT-PCR at the total RNA negative control group, sequenced, isolated and alignment in the NCBI gene bank. (2) the same cDNA fragment gene of hyaluronidase mice testis (from the negative control group) did not transcript in the treatment I and positive control groups (where there is no band), but this gene will be transcripted in the treatment II group (where the band is emerged). Key words: Male contraception, gandarusa, hyaluronidase gene, PCR analysis

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine > Medicine Study Program
Depositing User:	Sulimin BP3
Date Deposited:	06 Sep 2023 09:52
Last Modified:	06 Sep 2023 09:52
URI:	http://erepository.uwks.ac.id/id/eprint/15377

Actions (login required)

View Item

Downloads