The Effect of Ethanolic Extract of Mangosteen Peel As Adjuvant Therapy to TNF-Α And IL-10 in Wistar Rats Infected with M.tuberculosis H37rv

WIDYANINGSIH, INDAH (2019) The Effect of Ethanolic Extract of Mangosteen Peel As Adjuvant Therapy to TNF-Α And IL-10 in Wistar Rats Infected with M.tuberculosis H37rv. Journal of Global Pharma Technology-India. (Unpublished)

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Abstract

Abstract Objective: This study was an experimental study of wistar rats infected with M. tuberculosis H37 Rv and then given isoniazid INH as an anti-tuberculosis drug and ethanolic extract of mangosteen peel as adjuvant therapy. Method: Rats infected with M tuberculosis H37rv 106 Mc Farland, as 100µl intratracheal. Rats were randomly divided into six groups control (K1), rats infected with M tuberculosis H37rv only (K2), and rats infected with M tuberculosis H37rv and then treated with INH 100,200, 300 and 400 mg/kg body weight/ day (P1, P2, P3 and P4, respectivelyAfter administration of 4 weeks of therapy, the rats were sacrificed and the right lung tissue was collected to measured the levels of TNF α and IL 10 by using ELISA. Results The mean level of TNF α was found in the lowest P2 group compared to the treatment group (P1, P3 and P4) and the control was 24.16pg/ml. The level of IL 10 was found in the lowest P2 group compared to the treatment group (P1, P3 and P4) and the control was 232.75pg/ml. Ethanolic extract of mangosteen peel has a significant effect on decreasing levels of TNF α with a value of P = 0.000 <0.005 and IL 10 with a value of p = 0.001 <0.005. Conclusion Ethanolic extract of mangosteen peel is effective in reducing levels of TNF α and IL10 in adjuvant tuberculosis therapy, especially at doses of 200mg/kgbw/day, in smaller or larger doses there is an increased possibility of interaction between INH and the active substances in the extract. Therefore further studies are needed to find the cause. Keywords: M.tuberculosis, Mangosten, TNFα, IL10.

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine > Medicine Study Program
Depositing User:	Sulimin BP3
Date Deposited:	05 Sep 2023 05:21
Last Modified:	05 Sep 2023 05:21
URI:	http://erepository.uwks.ac.id/id/eprint/15317

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