Identification of BlaTEM and BlaSHV Genes of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Broilers Chicken in Blitar, Indonesia

Wibisono, Freshinta Jellia (2021) Identification of BlaTEM and BlaSHV Genes of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Broilers Chicken in Blitar, Indonesia. EManuscript Technologies-India. (Unpublished)

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Abstract

ABSTRACT The purpose of this research was to identify blaTEM and blaSHV genes of extended spectrum beta lactamase (ESBL) of Escherichia coli from cloacal swab of broiler chicken in several broiler farms in Blitar. This study used 95 broiler chicken samples, with cloacal swab method. The samples were isolated and identified to find Escherichia coli with several procedures, MacConckey Agar (MCA), Eosin Methylene Blue Agar (EMBA), Gram staining, indole test, Methyl Red-Voges Proskauer (MR-VP), citrate, and Triple Sugar Iron Agar (TSIA). ESBL-producing Escherichia coli bacteria isolated from cloacal swabs of broiler chicken were confirmed by the Double Disc Synergy Test (DDST). This confirmation test with DDST was conducted to evaluate the presence of inhibitory zones of ESBL activity with clavulanic acid using the Kirby-Bauer disk diffusion method on MuellerHinton agar (Merck, Germany). Double Disc Synergy Test uses the antibiotic disc Amoxycillin-clavulanic 30μg (Oxoid, England), Cefotaxime 30μg (Oxoid, England), Ceftazidime 30μg (Becton Dickinson, USA and Aztreonam 30μg (Oxoid, England). ESBL producing E. coli were 10 isolates and blaTEM gene from 7 isolates and blaSHV gene was no isolate. It can be explained that broiler chicken should be considered as a source of transmission for ESBL of E. coli to the public health. Keywords: Escherichia coli, blaTEM, blaSHV, ESBL, Broiler chicken, Public health

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Vetenary Medicine > Vetenary Education Study Program
Depositing User:	Sulimin BP3
Date Deposited:	03 Apr 2023 05:11
Last Modified:	03 Apr 2023 05:11
URI:	http://erepository.uwks.ac.id/id/eprint/14165

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