Uji toksisitas akut ekstrak daun seledri (Apium graveolens L) terhadap histopatologi ginjal tikus puth Sprague dawley

Pitaloka, Anastasia Rani (2022) Uji toksisitas akut ekstrak daun seledri (Apium graveolens L) terhadap histopatologi ginjal tikus puth Sprague dawley. Bachelor (S1) thesis, Wijaya Kusuma Surabaya University.

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Abstract

ABSTRAK Penelitian ini dilakukan untuk mengetahui efek toksisitas akut ekstrak daun seledri (Apium graveolens L) terhadap histopatologi ginjal tikus putih Sprague dawley. Sebanyak 24 sampel tikus putih dibagi ke dalam 4 kelompok perlakuan dan masing�masing kelompok perlakuan terdiri dari 6 ekor tikus putih jantan yang sehat. Ekstrak seledri yang digunakan dibagi ke dalam 4 dosis, yaitu dosis kontrol dan dosis perlakuan yaitu 50 mg/kg BB, 500 mg/kg BB dan 5000 mg/kg BB. Pemberian perlakuan ekstrak seledri sebanyak 1 ml diberikan selama 14 hari menggunakan alat pencekok oral pada tikus. Tikus dieuthanasia menggunakan metode servikal dislokasi. Pewarnaan preparat menggunakan teknik Heamatoxylin Eosin. Parameter yang digunakan adalah nekrosis, inflamasi, degenerasi, hemoragi dan kongesti. Histopatologi ginjal diamati dibawah mikroskop dengan perbesaran 400x. Analisis data menggunakan uji stastistik Kruskal Wallis menunjukkan bahwa hasil penelitian dengan pemberian ekstrak seledri dosis normal 0 dan dosis rendah 50 mg/kg BB tidak memberikan efek toksisitas akut, sedangkan dosis sedang 500 mg/kg BB dan dosis tinggi 5000 mg/kg BB memberikan efek tosksisitas akut terhadap histopatologi ginjal tikus putih Sprague Dawley. Kata kunci : toksisitas akut, seledri (Apium graveolens L), ginjal, tikus putih Sprague dawley ABSTRACT This study was conducted to determine the effect of acute toxicity of celery leaf extract (Apium graveolens L) on the kidney histopathology of white rats Sprague Dawley. A total of 24 samples of white rats were divided into 4 treatment groups and each treatment group consisted of 6 healthy male white rats. The celery extract used was divided into 4 doses, namely the control dose and the treatment dose, 50 mg/kg BW, 500 mg/kg BW and 5000 mg/kg BW. The treatment with 1 ml of celery extract was given for 14 days using an oral feeder on rats. Rats were euthanized using the cervical dislocation method. Staining preparations using the technique Haematoxylin Eosin. The parameters used are necrosis, inflammation, degeneration, hemorrhage and congestion. Kidney histopathology was observed under a microscope with a magnification of 400x. Data analysis using statistical Kruskal Wallis test showed that the results of the study with normal doses of celery extract 0 and a low dose of 50 mg/kg BW did not give an acute toxic effect, while a moderate dose of 500 mg/kg BW and a high dose of 5000 mg/kg BW gave an acute toxicological effect on the kidney histopathology of white rats Sprague Dawley. Keywords:acute toxicity, celery (apium graveolens L), kidney, white rat Sprague dawley

Item Type:	Thesis (Bachelor (S1))
Uncontrolled Keywords:	Kata kunci : toksisitas akut, seledri (Apium graveolens L), ginjal, tikus putih Sprague dawley Keywords:acute toxicity, celery (Apium graveolens L), kidney, white rat Sprague dawley
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Vetenary Medicine
Depositing User:	anastasia rani pitaloka
Date Deposited:	27 Sep 2022 02:10
Last Modified:	27 Sep 2022 02:10
URI:	http://erepository.uwks.ac.id/id/eprint/12439

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