Gambaran Histopatologi Ginjal dan Jumlah Sel Monosit Tikus Putih (Rattus norvegicus) dengan Pemberian Ekstrak Daun Kembang Bulan (Tithonia diversifolia) dan Metformin

Ningsih, Awalul Fitria (2022) Gambaran Histopatologi Ginjal dan Jumlah Sel Monosit Tikus Putih (Rattus norvegicus) dengan Pemberian Ekstrak Daun Kembang Bulan (Tithonia diversifolia) dan Metformin. Bachelor (S1) thesis, Wijaya Kusuma Surabaya University.

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Abstract

ABSTRAK Penelitian ini dilakukan untuk mengetahui gambaran histopatologi ginjal dan jumlah sel monosit tikus putih (Rattus norvegicus) dengan pemberian ekstrak daun kembang bulan (Tithonia diversifolia) dan metformin. Menggunakan 24 ekor tikus wistar jantan dengan 4 perlakuan yaitu P0 kontrol negatif diberikan makan dan minum, PI di induksi aloksan 100 mg/kg BB dan metformin 250 mg/kg BB, P2 kontrol positif di induksi aloksan 100 mg/kg BB, P3 dengan induksi aloksan 100 mg/kg BB dan pemberian ekstrak daun kembang bulan 300 mg/kg BB. Induksi aloksan secara intraperitoneal dan pemberian ekstrak serta metformin menggunakan sonde lambung. Tikus dieuthanasi secara inhalasi dan dilakukan pembedahan secara laparotomi. Sampel organ yang dikoleksi adalah ginjal bagian dexter yang kemudian dijadikan preparat histopatologi dengan pewarnaan hematoksilin eosin (HE). Lesi yang diamati berupa sel radang dan nekrosis menggunakan mikroskop binokuler dengan perbesaran 40x pada lima lapang pandang. Sampel darah dilakukan pewarnaan menggunakan giemsa untuk mengamati jumlah sel monosit dengan perbesaran mikroskop 100x. Hasil skoring lesi sel radang dan nekrosis dianalisis dengan uji statistik Kruskal-Wallis yang menujukkan terdapat perbedaan nyata (P<0,05), maka dilanjutkan dengan uji Mann-Withney juga menunjukkan perbedaan nyata kecuali pada kelompok P0 dengan P1 dan P1 dengan P3. Hasil uji One Way ANOVA pada hapusan darah sel monosit menujukkan adanya perbedaan nyata (P<0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa induksi aloksan dan pemberian esktrak daun kembang bulan serta metformin dapat mempengaruhi gambaran histopatologi organ ginjal dan sel monosit dalam darah. Kata kunci: Ekstrak daun kembang bulan, metformin, sel radang, nekrosis, sel monosit. ABSTRACT This study was conducted to determine the histopathological of the kidney and the number of monocyte cells in wistar rats (Rattus norvegicus) by administering flower moon leaf extract (Tithonia diversifolia) and metformin. Using 24 male wistar rats with 4 treatments, P0 negative control was given food and drink, PI was induced by alloxan 100 mg/kg BW and metformin 250 mg/kg BW, P2 positive control was induced alloxan 100 mg/kg BW, P3 induced alloxan 100 mg/kg BW and administration of flower moon leaf extract 300 mg/kg BW. Induction of alloxan intraperitoneally and administration of extracts and metformin using a gastric probe. Mice were euthanized by inhalation and surgically performed laparotomy. The organ samples collected were the right kidney which was then used as histopathological preparations with hematoxylin eosin (HE) staining. The observed lesions were inflammatory cells and necrosis using a binocular microscope with a magnification of 40x in five fields of view. Blood samples were stained using Giemsa to observe the number of monocytes with a microscope magnification of 100x. The results of scoring of inflammatory and necrotic cell lesions were analyzed by the Kruskal-Wallis statistical test which showed a significant difference (P<0.05), then followed by the Mann-Withney test which also showed significant differences except in the P0 group with P1 and P1 with P3. The results of the One Way ANOVA test on monocyte cell blood smears showed a significant difference (P<0.05). Based on the results of the study, it can be concluded that the induction of alloxan and administration of flower moon leaf extract and metformin can affect the histopathological picture of kidney organs and monocyte cells in the blood. Keywords: Moonflower leaf extract, metformin, inflammatory cells, necrosis, monocyte cells.

Item Type:	Thesis (Bachelor (S1))
Uncontrolled Keywords:	Ekstrak daun kembang bulan, metformin, sel radang, nekrosis, sel monosit.
Subjects:	R Medicine > R Medicine (General) R Medicine > RB Pathology R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RZ Other systems of medicine
Divisions:	Faculty of Vetenary Medicine > Vetenary Education Study Program
Depositing User:	82 Awalul Fitria Ningsih UWKS
Date Deposited:	21 Sep 2022 03:47
Last Modified:	21 Sep 2022 03:47
URI:	http://erepository.uwks.ac.id/id/eprint/12286

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